Aim of study: The aim of study was to evaluate antiasthmatic activity of various extracts of Peganum harmala seeds to validate its traditional use. Materials and methods: In the present study petroleum ether, ethanol and water extract of Peganum harmala seeds at the doses of 25-100 mg/kg i. p. was evaluated for antiasthmatic activity using clonidine induced mast cells degranulation in mice. Results: Treatment group of PEEE (Peganum harmala ethanol extract) exhibited significant mast cell stabilizing potential at 100 mg/kg i.p 68.85±1.30 as compared to control group. Percent protection offered by PHEE at 100 mg/kg was 20.28% and was of statistical significance as compared to PHPEE and PHWE. Conclusion: It can be concluded that the ethanol extract of Peganum harmala (PHEE) may be useful in management of asthma.