Ethnopharmacological relevance: seeds of Anethum graveolens have been used in the Indian traditional medicine to relieve stress and other neurological disease conditions. Aim of the study: The present study was under taken to evaluate the anxiolytic effects of seeds of Anethum graveolens Ethanolic Extract (AGEE) in mice. Materials and methods: seeds of Anethum graveolens Ethanolic extract was screened for anxiolytic effect by using using locomotor activity at doses of 100 and 200 mg/kg. Distilled water and Diazepam were employed as negative and positive control groups, respectively. Results: anxiolytic effect assessment of seeds of Anethum graveolens Ethanolic Extract (AGEE) 200 mg/kg shows significantly decrease in locomotion (45.66 %) as compared to standard (69.05 %) treatment group. Conclusion: The results of this study established a support for the traditional usage of seeds of Anethum graveolens as anxiolytic medicinal plant.